In vitro degradation of natural insoluble lignin in aqueous media by the extracellular peroxidases ofPhanerochaete chrysosporium

To improve the conventional and commercially-available medical adhesives such as cyanoacrylate, aldehyde-based, and fibrin glue, new bioadhesive has been prepared using medical and food additives as starting materials. Aldehyde groups could be easily introduced in dextran in the presence of sodium periodate in aqueous media, and the extent of the introduction could also be controlled. In vitro degradation speed of the hydrogel prepared by mixing of aldehyded dextran with ε-poly(L-lysine) at 37oC significantly varied by acetic anhydride concentration added to ε-poly(L-lysine) from < 5h to > 5 weeks. Bonding strength of the glue was 4 times higher than that of commercial fibrin glue and almost no cytotoxicity was observed, suggesting the development of novel self-degradable bioadhesive.

Download Full-text

In vitro degradation of insoluble lignin in aqueous media by lignin peroxidase and manganese peroxidase

Applied Biochemistry and Biotechnology ◽

10.1007/bf02920207 ◽

1998 ◽

Vol 70-72 (1) ◽

pp. 967-982 ◽

Cited By ~ 5

Author(s):

David N. Thompson ◽

Bonnie R. Hames ◽

C. A. Reddy ◽

Hans E. Grethlein

Keyword(s):

Lignin Peroxidase ◽

Manganese Peroxidase ◽

Aqueous Media ◽

In Vitro Degradation

Download Full-text

In Vitro Degradation Of beta-Amyloid[25-35] Peptide

Protein and Peptide Letters ◽

10.2174/0929866013409076 ◽

2001 ◽

Vol 8 (6) ◽

pp. 423-428 ◽

Cited By ~ 1

Author(s):

Krisztina Jost ◽

Jozsef Varga ◽

Botond Pence ◽

Marta Zarandi

Keyword(s):

Beta Amyloid ◽

In Vitro Degradation

Download Full-text

In Vitro Degradation of Absorbable Zinc Alloys in Artificial Urine

Materials ◽

10.3390/ma12020295 ◽

2019 ◽

Vol 12 (2) ◽

pp. 295 ◽

Cited By ~ 11

Author(s):

Sébastien Champagne ◽

Ehsan Mostaed ◽

Fariba Safizadeh ◽

Edward Ghali ◽

Maurizio Vedani ◽

...

Keyword(s):

Surface Characterization ◽

Urinary Tract Obstruction ◽

Calcium Content ◽

Corrosion Layer ◽

Uniform Corrosion ◽

In Vitro Degradation ◽

Pure Zinc ◽

Zinc Alloys ◽

Artificial Urine

Absorbable metals have potential for making in-demand rigid temporary stents for the treatment of urinary tract obstruction, where polymers have reached their limits. In this work, in vitro degradation behavior of absorbable zinc alloys in artificial urine was studied using electrochemical methods and advanced surface characterization techniques with a comparison to a magnesium alloy. The results showed that pure zinc and its alloys (Zn–0.5Mg, Zn–1Mg, Zn–0.5Al) exhibited slower corrosion than pure magnesium and an Mg–2Zn–1Mn alloy. The corrosion layer was composed mostly of hydroxide, carbonate, and phosphate, without calcium content for the zinc group. Among all tested metals, the Zn–0.5Al alloy exhibited a uniform corrosion layer with low affinity with the ions in artificial urine.

Download Full-text

Improved Antioxidant Capacity of Black Tea Waste Utilizing PlantCrystals

Molecules ◽

10.3390/molecules26030592 ◽

2021 ◽

Vol 26 (3) ◽

pp. 592 ◽

Cited By ~ 1

Author(s):

Abraham M. Abraham ◽

Reem M. Alnemari ◽

Jana Brüßler ◽

Cornelia M. Keck

Keyword(s):

Antioxidant Capacity ◽

Aqueous Media ◽

Natural Antioxidants ◽

Black Tea ◽

Pharmaceutical Products ◽

Small Scale ◽

Alternative Source ◽

Significant Difference ◽

Plant Waste

Antioxidants are recommended to prevent and treat oxidative stress diseases. Plants are a balanced source of natural antioxidants, but the poor solubility of plant active molecules in aqueous media can be a problem for the formulation of pharmaceutical products. The potential of PlantCrystal technology is known to improve the extraction efficacy and antioxidant capacity (AOC) of different plants. However, it is not yet proved for plant waste. Black tea (BT) infusion is consumed worldwide and thus a huge amount of waste occurs as a result. Therefore, BT waste was recycled into PlantCrystals using small-scale bead milling. Their characteristics were compared with the bulk-materials and tea infusion, including particle size and antioxidant capacity (AOC) in-vitro. Waste PlantCrystals possessed a size of about 280 nm. Their AOC increased with decreasing size according to the DPPH (1,1-diphenyl-2-picrylhydrazyl) and ORAC (oxygen radical absorbance capacity) assays. The AOC of the waste increased about nine-fold upon nanonization, leading to a significantly higher AOC than the bulk-waste and showed no significant difference to the infusion and the used standard according to DPPH assay. Based on the results, it is confirmed that the PlantCrystal technology represents a natural, cost-effective plant-waste recycling method and presents an alternative source of antioxidant phenolic compounds.

Download Full-text